BCHE 8210 - Fermentation Engineering Laboratory (Microbial Physiology and Bioprocess Engineering)


Spring Semester 2025

Course Instructor

Dr. Mark A. Eiteman
542-0833
112 Riverbend South
eiteman@engr.uga.edu
email is a terrific way to communicate with me!

Meeting Times

Wednesday, Friday
9:10 - 10:00
1367 Driftmier
Three labs will be scheduled during the semester. These will vary in their length and schedule. Lab 1 will require a three hour block of time; Lab 2 will require students to schedule a 15 minute sampling time throughout a day' Lab 3 will take three days, but the actual sampling will be a single time.
Students will also be responsible for setting up and conducting an independent "lab experiment". The goal of this lab experiment will be to develop and implement a lab that would be suitable for undergraduate students (e.g., as part of 'Advanced Biochemical Engineering' - 4180L).

Description of course

This lab/lecture discussion course will cover microbial cell physiology and bioreactor operational modes (i.e., batch, fed-batch, chemostat). How do microbes behave under different conditions? How do cells modify their behavior in response to certain example gene knockouts? How does one quantify microbial behavior? The course will also assist students in designing microbial fermentation experiments and interpreting results. The focus will be bacteria. Although E. coli will be used as the principal example, some discussion will be on other species.

Detailed syllabus

AssignmentsDue Date
Problem #1Wednesday, January 22
Problem #2Friday, January 24
  • Jantama et al. 2008
  • Problem #3Friday, January 31
  • Wang et al. 2018
  • Problem #5Friday, February 14
    Problem #6Friday, February 21
    ----------------------------------------------------------------------
    Critique #1Wednesday, February 5
  • Martinez et al. 2008
  • Centeno-Leija et al. 2013
  • Centeno-Leija et al. 2014
  • Critique #2
  • Guevara-Martinez et al. 2015
  • Masuda et al. 2017
  • Perez-Zabaleta et al. 2019
  • ----------------------------------------------------------------------
    Lab #1Monday, February 10, 5:00 - 7:30
    Report DueTBA
  • Lab #1 Record
  • Lab #2Tuesday, March 11 (all day)
  • Lab #2 Record
  • Lab #2 Medium
  • Report DueTBA
    Lab #3
  • Lab #3 Record
  • Report DueTBA
    Independent Project
  • Zupan et al 2004
  • Report DueMonday, May 5
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    Video on Mass Transfer Theory
    Video on Oxygen Transfer Rate
    Video on Measurement of kLa

    Solutions

    Some Useful Enzyme Links
    Brenda
    Enzyme Nomenclature
    GenomeNet

    MATLAB Examples
    Example 1
    Example 2
    Example 3 - while loop
    Example 4a - for loop
    Example 4b - for loop with excel output
    The Excel output from Example 4b